Method Development for the processing and utilization of marine invasive species biomass

Duration Months 6-30

Objectives

This specific action is aimed at finding ways to further utilize the biomass of F. commersonii which is in great abundance according to the researches so far, in finding further applications of utilization of P.miles which according to the forecasts will acquire more and more abundances, but also in identifying the most effective techniques for “detoxification” of L.sceleratus biomass (ie removal of tetradotoxin TTX from tissues), in order to ensure alternative ways of utilizing its “pure” biomass in biotechnological applications which may arise (eg production of high nutritional value fertilizer). Technological equipment (High Performance Liquid Chromatography – Tandem Mass Spectrometry, LC-MS / MS) and modern chromatographic methods (Hydrophilic Interaction Liquid Chromatography, HIL) will be used to perform the measurements, which will allow the quality determination of both TTX and of its possible chemical transformation products.

WP leader: HCMR

Actions

• Development of techniques for separation-isolation of active substances from fishery samples

  • Research and development of treatment techniques for the detoxification of L.sceleratus biomass
  • Isolation of omega-3 fatty acids from the pure biomass of L.sceleratus and P.miles. Collagen production techniques from the bones of the two toxic species.

• Techniques for the production of fatty acids and collagen from F. commersonii

• Development of techniques for separation-isolation of toxins from the two toxic species

• Exploring alternative ways of utilizing toxins in other biotechnological applications that may arise (eg insect repellents, analgesics)